![]() megaterium belongs to one of the biggest known bacteria. 1 With a cell length of up to 4 µm and a diameter of 1.5 µm, B. In 1884 de Bary named the Gram-positive bacterium Bacillus megaterium the “big beast” due to its large size. All these features provide a solid basis for challenging biotechnological approaches. megaterium invites for cell biology research projects. All data can be integrated by using a novel bioinformatics platform. Proteome, fluxome and metabolome analyses are possible. For further system biotechnology based optimization strategies the genome sequence will provide a closer look into genomic capacities of B. For this purpose, the overexpression of chromosomal and plasmid encoded genes and operons, the synthesis of anti-sense RNA for gene silencing, the removal of inhibitory regulatory elements in combination with the utilization of strong promoters, directed protein design, and the recombinant production of B 12 binding proteins to overcome feedback inhibition were successfully employed. megaterium served as a model for the systematic development of a production strain using these tools. Vectors for chromosomal integration enable the direct manipulation of the genome. On the basis of two compatible vectors, a T7 RNA polymerase driven protein production system was established. For the purpose of intra- as well as extracellular protein synthesis several vectors were constructed and commercialized (MoBiTec GmbH, Germany). During the last years, it became more and more popular in the field of biotechnology for its recombinant protein production capacity. ![]() Bacillus megaterium, the “big beast,” is a Gram-positive bacterium with a size of 4 × 1.5 µm. ![]()
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